ly255283  (R&D Systems)

Journal: Cell Reports Medicine

Article Title: LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis

doi: 10.1016/j.xcrm.2025.102000

Figure Lengend Snippet:

Article Snippet: The next day, the cells were treated with DEN (25 ng/mL), LTB4 (100 nM, Cayman, 20110) and inhibitors including JNK-IN-8 (2.5 μM, Aladdin, J125321), LY255283 (5 μM, MCE, HY-15744) and U-75302 (200 nM, Cayman, 70705), as indicated in the figures.

Techniques: Microarray, Recombinant, Lysis, Protease Inhibitor, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Reverse Transcription, Activity Assay, Immunoprecipitation, Extraction, Purification, In Vitro, Transfection, Sequencing, Mass Cytometry, ChIP-sequencing, Transgenic Assay, Software

Journal: iScience

Article Title: A LTB 4 /CD11b self-amplifying loop drives pyogranuloma formation in chronic granulomatous disease

doi: 10.1016/j.isci.2024.109589

Figure Lengend Snippet:

Article Snippet: Where indicated, cells were plated in the presence of the following antagonists or antibodies at the time of plating: 8 μg/mL U-75302, 83.3 μM LY255283, 5 μM reparixin L-lysine, 1 μM Boc-MLF, 1 μM cyclosporin H, 1 μM WRW4, 10 μg/mL anti-CD11b (clone 5C6), 10 μg/mL anti-CCL2 (clone 2H5), 10 μg/mL anti CCL3 (polyclonal, R&D Systems), and 10 μg/mL anti-CCL4 (polyclonal, R&D Systems).

Techniques: Recombinant, Sequencing, Enzyme-linked Immunosorbent Assay, Software